Publications

*Undergraduate students

  1. Carr, S.C., Aldridge, J., and N.R. Buan. 2021. Isoprene production from municipal wastewater biosolids by engineered archaeaon Methanosarcina acetivorans. Appl Sci. 11(8), 3342. DOI:10.3390/app11083342.
  2. Aldridge, J., Carr, S., Weber, K.A., and N.R. Buan. 2021. Anaerobic production of isoprene by engineered Methanosarcina spp. archaea. Appl Environ Microbiol. 87(6) e02417-20. DOI: 10.1128/AEM.02417-20.
  3. Catlett, J.L., Catazaro, J., Cashman, M., Carr, S.C., Powers, R., Cohen, M.B., and N.R. Buan. 2020. Metabolic feedback inhibition influences metabolite secretion by the human gut symbiont Bacteroides thetaiotaomicron. mSystems. 5(5) e00252-20. DOI: 10.1128/mSystems.00252-20.
  4. Buan, N.R., Weber, K.A., Aldridge, J.T., and Carr, S.C. 2018. Production of bioisoprene from wastewater. Water Research Foundation. ISBN: 978-1-94124-296-4.
  5. Buan, N.R. 2018. Methanogens: Pushing the boundaries of Biology. Emerging Topics in Archaeal Biology. Biochemical Society/Portland Press. 2: 629–646. DOI: 10.1042/ETLS20180031.
  6. Duszenko*, N., and N.R. Buan. 2017. Physiological evidence for isopotential tunneling in the electron transport chain of methane-producing archaea. Appl Environ Microbiol. 83(18) e00950-17. DOI: 10.1128/AEM.00950-17. PMID: 28710268.
  7. Sakkaff, Z., Catlett, J.L., Cashman, M., Pierobon, M., Buan, N.R., Cohen, M.B., and C.A. Kelley. 2017. End-to-end Molecular Communication Channels in Cell Metabolism: an Information Theoretic Study. ACM NanoCom. DOI: 0.1145/3109453.3109474.
  8. Cashman, M., Catlett, J. L., Cohen, M. B., Buan N. R., Sakkaff, Z., Pierobon, M., and C. Kelley. 2017. BioSIMP: Using Software Testing Techniques for Sampling and Inference in Biological Organisms. 12th International Workshop on Software Engineering for Science (SE4Science). DOI: 10.1109/SE4Science.2017..9.
  9. Walter*, M.E., Ortiz*, A., Sondgeroth*, C., Sindt*, N., Duszenko*, N., Anderson, C., N., Fernando, S., and N.R. Buan. 2016. High-throughput mutation, selection, and phenotype screening of methanogenic archaea. J Microbiol Methods. 131: 113-121. PMID: 27771305.
  10. Pierobon, M., Sakkaff, Z., Catlett, J.L., Buan, N.R. 2016. Mutual Information Upper Bound of Molecular Communication Based on Cell Metabolism. IEEE 17th International Workshop on Signal Processing Advances in Wireless Communications (SPAWC 2016). DOI: 10.1109/SPAWC.2016.7536835.
  11. Cashman, M., Catlett, J.L., Cohen, M.B., Buan, N., Sakkaff, Z., Pierobon, M., and C. Kelley. 2016. Sampling and Inference in Configurable Biological Systems: A Software Testing Perspective. Computer Science & Engineering, University of Nebraska-Lincoln, Technical Report #TR-UNL-CSE-2016-0007.
  12. Aldridge, J.T., Catlett, J.L., Smith*, M.L., and N. R. Buan. 2016. Methods for detecting methane production and consumption by gas chromatography. Bio-Protocol. 6(7): e1779. http://www.bio-protocol.org/e1779. PMID: 27771305.
  13. Shea*, M.T., Walter*, M.E., Duszenko*, N., Ducluzeau, A.-L., Aldridge, J., King*, S.K. and N.R. Buan. 2016. pNEB193-derived suicide plasmids for gene deletion and protein expression in the methane-producing archaeon, Methanosarcina acetivorans. Plasmid. doi:10.1016/j.plasmid.2016.02.003. PMID: 26876941.
  14. Pierobon, M., Cohen, M.B., Buan, N. and Kelley, C., SCIM: Sampling, Characterization, Inference and Modeling of Biological Consortia, Sep. 2015, Computer Science & Engineering, University of Nebraska-Lincoln, Technical Report #TR-UNL-CSE-2015-0002, 2015.
  15. Catlett, J.L., Ortiz*, A.M., and N.R. Buan. 2015. Rerouting cellular electron flux to increase the rate of biological methane production. Appl Environ Microbiol. 81(19):6528-37. PMID: 26162885.
  16. Lieber*, D. J., Catlett, J., Madayiputhiya, N., Nandukumar, R., Lopez, M.M., Metcalf, W.M., and N.R. Buan. 2014. A multienzyme complex channels substrates and electrons through acetyl-CoA and methane biosynthesis pathways in Methanosarcina. PLOS ONE. 9: e107563. doi:10.1371/journal.pone.0107563. PMID: 25232733.
  17. Buan, N.R., Kulkarni, G., and W.W. Metcalf. 2011. Genetic methods for Methanosarcina species. In: Rosenzweig, A. and S. Ragsdale, eds. Methods in Methane Metabolism (Methods in Enzymology). 494:23-42. PMID: 25232733.
  18. Buan, N.R. and W.W. Metcalf. 2010. Methanogenesis by Methanosarcina acetivorans involves two structurally and functionally distinct classes of heterodisulfide reductase. Mol. Microbiol. 75:843-53. PMID: 19968794.
  19. Escalante-Semerena, J.C., Woodson, J.D., Buan, N.R., and C.L. Zayas. Conversion of cobinamide into coenzyme B12. In: Warren M.J., Smith A.G., eds. Tetrapyrroles: Birth, Life and Death. Austin/New York: Landes Bioscience/Springer Science+Business Media, 2009:300-16.
  20. Ruiz-Sánchez, P., Mundwiler, S., Spingler, B., Buan, N. R., Escalante-Semerena, J. C., and R. Alberto. 2008. Syntheses and characterization of vitamin B12-Pt(II) conjugates and their adenosylation in an enzymatic assay. J. Biol. Inorg. Chem. 13:335-47.
  21. Buan, N. R. and J. C. Escalante-Semerena. 2006. Purification and initial biochemical characterization of the ATP:cob(I)alamin adenosyltransferase (EutT) enzyme of Salmonella enterica. J. Biol. Chem. 281:16971-77.
  22. Buan, N. R., Rehfeld, K.*, and J. C. Escalante-Semerena. 2006. Studies of the CobA-type ATP:Co(I)rrinoid Adenosyltransferase enzyme of Methanosarcina mazei strain Gö1. J. Bacteriol. 188: 3543-50.
  23. Buan, N. R. and J. C. Escalante-Semerena. 2005. Computer-assisted docking of flavodoxin with the ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme reveals residues critical for protein-protein interactions but not for catalysis. J. Biol. Chem. 280: 40948-56.
  24. Stich, T. A., Buan, N. R., Escalante-Semerena, J. C., and T. C Brunold. 2005. Spectroscopic and Computational studies of the ATP:corrinoid adenosyltransferase (CobA) from Salmonella enterica: insights into the mechanism of adenosylcobalamin biosynthesis. J. Am. Chem. Soc. 127: 8710-19.
  25. Stich T. A., Buan N. R., and T. C. Brunold. 2004. Spectroscopic and computational studies of Co2+corrinoids: spectral and electronic properties of the biologically relevant base-on and base-off forms of Co2+cobalamin. J. Am. Chem. Soc. 126: 9735-49.
  26. Buan N. R., Suh S.-J., and J. C. Escalante-Semerena.  2004. The eutT gene of Salmonella enterica encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme. J. Bacteriol. 186: 5708-14.
  27. Basha, E., Lee, G. J., Breci, L. A., Hausrath, A. C., Buan*, N. R., Giese, K. C., and E. Vierling. 2004. The identity of proteins associated with a small heat shock protein during heat stress in vivo indicates that these chaperones protect a wide range of cellular functions. J. Biol. Chem. 279: 7566-75.
  28. Friedrich, K. L., Giese, K. C., Buan*, N. R., and E. Vierling. 2004. Interaction between small heat shock protein subunits and substrate in small heat shock protein-substrate complexes. J. Biol. Chem. 279: 1080-89.
  29. Stich T. A., Brooks, A. J., Buan, N. R., and T. C. Brunold. 2003. Spectroscopic and computational studies of Co3+-corrinoids: spectral and electronic properties of the B12 cofactors and biologically relevant precursors. J. Am. Chem. Soc. 125: 5897-5914.
  30. Fonseca M. V., Buan, N. R., Horswill, A. R., Rayment, I.  and J. C. Escalante-Semerena. 2002. The ATP:co(I)rrinoid adenosyltransferase (CobA) enzyme of Salmonella enterica requires the 2’-OH group of ATP for function and yields inorganic triphosphate as its reaction byproduct. J. Biol. Chem. 277: 33127-31.

Patents

  1. Aldridge, J., Carr, S., Weber, K.A., and N.R. Buan. 2016. “Production of Isoprene by Methane-producing Archaea”. US patent US20170175145 A1.
  2. Catlett, J.L. and N.R. Buan. 2015. “Microbial strains and methods for making and using”. US patent US20150299673 A1.