*Undergraduate students

  1. Brown, J., Hines, C., Rodrigues de Queiroz, A., Sahay, S., Vijayan, J., Stone, J.M., Bickford, N., Wuellner, M., Glowacka, K., Buan, N.R., and R.L. Roston. The Effects of Exogenously Applied Antioxidants on Plant Growth and Resilience. Phyt Rev. doi: 10.1007/s11101-023-09862-3.
  2. Salvi*, A.M., Chowdhury, N.B., Saha, R., and N.R. Buan. 2023. Supplementation of sulfide or acetate and 2-mercaptoethane sulfonate restores growth of the Methanosarcina acetivorans ΔhdrABC deletion mutant during methylotrophic methanogenesis. Microorganisms. 11(2), 327.
  3. Carr, S. and N.R. Buan. 2022. Insights into the biotechnology potential of Methanosarcina. Frontiers in Microbiology.
  4. Catlett, J.L., Carr, S.C., Cashman, M., Smith*, M., Walter*, M.E., Sakkaff, Z., Kelley, C., Pierobon, M., Cohen, M.B., and N.R. Buan. Metabolic synergy between human symbionts Bacteroides and Methanobrevibacter. Microbiol Spectr. 2022 May 10:e0106722. doi: 10.1128/spectrum.01067-22. PMID: 35536023.
  5. Hoke, K.L., Zimmer, S.L., Roddy, A.B., Ondrechen, M.J., Williamson, C.E., and N. R. Buan. 2022. Reintegrating Biology Through the Nexus of Energy, Information and Matter. 2022. Integr Comp Biol. Feb 5;61(6):2082-2094. doi: 10.1093/icb/icab174. PMID: 34374780. Authorship determined by random order.
  6. White, K., McEntire, K., Buan, N.R., Robinson, L., and E. Barbar. 2022. Charting a new frontier integrating mathematical modeling in complex biological systems from molecules to ecosystems. Integr Comp Biol. Feb 5;61(6):2255-2266. doi: 10.1093/icb/icab165. PMID: 34283225. Authorship determined by random order.
  7. Carr, S.C., Aldridge, J., and N.R. Buan. 2021. Isoprene production from municipal wastewater biosolids by engineered archaeaon Methanosarcina acetivorans. Appl Sci. 11(8), 3342. DOI:10.3390/app11083342.
  8. Aldridge, J., Carr, S., Weber, K.A., and N.R. Buan. 2021. Anaerobic production of isoprene by engineered Methanosarcina spp. archaea. Appl Environ Microbiol. 87(6) e02417-20. DOI: 10.1128/AEM.02417-20.
  9. Catlett, J.L., Catazaro, J., Cashman, M., Carr, S.C., Powers, R., Cohen, M.B., and N.R. Buan. 2020. Metabolic feedback inhibition influences metabolite secretion by the human gut symbiont Bacteroides thetaiotaomicron. mSystems. 5(5) e00252-20. DOI: 10.1128/mSystems.00252-20.
  10. Buan, N.R., Weber, K.A., Aldridge, J.T., and Carr, S.C. 2018. Production of bioisoprene from wastewater. Water Research Foundation. ISBN: 978-1-94124-296-4.
  11. Buan, N.R. 2018. Methanogens: Pushing the boundaries of Biology. Emerging Topics in Archaeal Biology. Biochemical Society/Portland Press. 2: 629–646. DOI: 10.1042/ETLS20180031.
  12. Duszenko*, N., and N.R. Buan. 2017. Physiological evidence for isopotential tunneling in the electron transport chain of methane-producing archaea. Appl Environ Microbiol. 83(18) e00950-17. DOI: 10.1128/AEM.00950-17. PMID: 28710268.
  13. Sakkaff, Z., Catlett, J.L., Cashman, M., Pierobon, M., Buan, N.R., Cohen, M.B., and C.A. Kelley. 2017. End-to-end Molecular Communication Channels in Cell Metabolism: an Information Theoretic Study. ACM NanoCom. DOI: 0.1145/3109453.3109474.
  14. Cashman, M., Catlett, J. L., Cohen, M. B., Buan N. R., Sakkaff, Z., Pierobon, M., and C. Kelley. 2017. BioSIMP: Using Software Testing Techniques for Sampling and Inference in Biological Organisms. 12th International Workshop on Software Engineering for Science (SE4Science). DOI: 10.1109/SE4Science.2017..9.
  15. Walter*, M.E., Ortiz*, A., Sondgeroth*, C., Sindt*, N., Duszenko*, N., Anderson, C., N., Fernando, S., and N.R. Buan. 2016. High-throughput mutation, selection, and phenotype screening of methanogenic archaea. J Microbiol Methods. 131: 113-121. PMID: 27771305.
  16. Pierobon, M., Sakkaff, Z., Catlett, J.L., Buan, N.R. 2016. Mutual Information Upper Bound of Molecular Communication Based on Cell Metabolism. IEEE 17th International Workshop on Signal Processing Advances in Wireless Communications (SPAWC 2016). doi:10.1109/SPAWC.2016.7536835.
  17. Cashman, M., Catlett, J.L., Cohen, M.B., Buan, N., Sakkaff, Z., Pierobon, M., and C. Kelley. 2016. Sampling and Inference in Configurable Biological Systems: A Software Testing Perspective. Computer Science & Engineering, University of Nebraska-Lincoln, Technical Report #TR-UNL-CSE-2016-0007.
  18. Aldridge, J.T., Catlett, J.L., Smith*, M.L., and N. R. Buan. 2016. Methods for detecting methane production and consumption by gas chromatography. Bio-Protocol. 6(7): e1779. PMID: 27771305.
  19. Shea*, M.T., Walter*, M.E., Duszenko*, N., Ducluzeau, A.-L., Aldridge, J., King*, S.K. and N.R. Buan. 2016. pNEB193-derived suicide plasmids for gene deletion and protein expression in the methane-producing archaeon, Methanosarcina acetivorans. Plasmid. doi:10.1016/j.plasmid.2016.02.003. PMID: 26876941.
  20. Pierobon, M., Cohen, M.B., Buan, N. and Kelley, C., SCIM: Sampling, Characterization, Inference and Modeling of Biological Consortia, Sep. 2015, Computer Science & Engineering, University of Nebraska-Lincoln, Technical Report #TR-UNL-CSE-2015-0002, 2015.
  21. Catlett, J.L., Ortiz*, A.M., and N.R. Buan. 2015. Rerouting cellular electron flux to increase the rate of biological methane production. Appl Environ Microbiol. 81(19):6528-37. PMID: 26162885.
  22. Lieber*, D. J., Catlett, J., Madayiputhiya, N., Nandukumar, R., Lopez, M.M., Metcalf, W.M., and N.R. Buan. 2014. A multienzyme complex channels substrates and electrons through acetyl-CoA and methane biosynthesis pathways in Methanosarcina. PLOS ONE. 9: e107563. doi:10.1371/journal.pone.0107563. PMID: 25232733.
  23. Buan, N.R., Kulkarni, G., and W.W. Metcalf. 2011. Genetic methods for Methanosarcina species. In: Rosenzweig, A. and S. Ragsdale, eds. Methods in Methane Metabolism (Methods in Enzymology). 494:23-42. PMID: 25232733.
  24. Buan, N.R. and W.W. Metcalf. 2010. Methanogenesis by Methanosarcina acetivorans involves two structurally and functionally distinct classes of heterodisulfide reductase. Mol. Microbiol. 75:843-53. PMID: 19968794.
  25. Escalante-Semerena, J.C., Woodson, J.D., Buan, N.R., and C.L. Zayas. Conversion of cobinamide into coenzyme B12. In: Warren M.J., Smith A.G., eds. Tetrapyrroles: Birth, Life and Death. Austin/New York: Landes Bioscience/Springer Science+Business Media, 2009:300-16.
  26. Ruiz-Sánchez, P., Mundwiler, S., Spingler, B., Buan, N. R., Escalante-Semerena, J. C., and R. Alberto. 2008. Syntheses and characterization of vitamin B12-Pt(II) conjugates and their adenosylation in an enzymatic assay. J. Biol. Inorg. Chem. 13:335-47.
  27. Buan, N. R. and J. C. Escalante-Semerena. 2006. Purification and initial biochemical characterization of the ATP:cob(I)alamin adenosyltransferase (EutT) enzyme of Salmonella enterica. J. Biol. Chem. 281:16971-77.
  28. Buan, N. R., Rehfeld, K.*, and J. C. Escalante-Semerena. 2006. Studies of the CobA-type ATP:Co(I)rrinoid Adenosyltransferase enzyme of Methanosarcina mazei strain Gö1. J. Bacteriol. 188: 3543-50.
  29. Buan, N. R. and J. C. Escalante-Semerena. 2005. Computer-assisted docking of flavodoxin with the ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme reveals residues critical for protein-protein interactions but not for catalysis. J. Biol. Chem. 280: 40948-56.
  30. Stich, T. A., Buan, N. R., Escalante-Semerena, J. C., and T. C Brunold. 2005. Spectroscopic and Computational studies of the ATP:corrinoid adenosyltransferase (CobA) from Salmonella enterica: insights into the mechanism of adenosylcobalamin biosynthesis. J. Am. Chem. Soc. 127: 8710-19.
  31. Stich T. A., Buan N. R., and T. C. Brunold. 2004. Spectroscopic and computational studies of Co2+corrinoids: spectral and electronic properties of the biologically relevant base-on and base-off forms of Co2+cobalamin. J. Am. Chem. Soc. 126: 9735-49.
  32. Buan N. R., Suh S.-J., and J. C. Escalante-Semerena.  2004. The eutT gene of Salmonella enterica encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme. J. Bacteriol. 186: 5708-14.
  33. Basha, E., Lee, G. J., Breci, L. A., Hausrath, A. C., Buan*, N. R., Giese, K. C., and E. Vierling. 2004. The identity of proteins associated with a small heat shock protein during heat stress in vivo indicates that these chaperones protect a wide range of cellular functions. J. Biol. Chem. 279: 7566-75.
  34. Friedrich, K. L., Giese, K. C., Buan*, N. R., and E. Vierling. 2004. Interaction between small heat shock protein subunits and substrate in small heat shock protein-substrate complexes. J. Biol. Chem. 279: 1080-89.
  35. Stich T. A., Brooks, A. J., Buan, N. R., and T. C. Brunold. 2003. Spectroscopic and computational studies of Co3+-corrinoids: spectral and electronic properties of the B12 cofactors and biologically relevant precursors. J. Am. Chem. Soc. 125: 5897-5914.
  36. Fonseca M. V., Buan, N. R., Horswill, A. R., Rayment, I.  and J. C. Escalante-Semerena. 2002. The ATP:co(I)rrinoid adenosyltransferase (CobA) enzyme of Salmonella enterica requires the 2’-OH group of ATP for function and yields inorganic triphosphate as its reaction byproduct. J. Biol. Chem. 277: 33127-31.


  1. Aldridge, J., Carr, S., Weber, K.A., and N.R. Buan. 2016. “Production of Isoprene by Methane-producing Archaea”. US patent US20170175145 A1.
  2. Catlett, J.L. and N.R. Buan. 2015. “Microbial strains and methods for making and using”. US patent US20150299673 A1.

Other products

  1. Engineering Biology Research Consortium. 2023. An Assessment of Short-Term Milestones in EBRC’s 2019 Roadmap, Engineering Biology.
  2. Engineering Biology Research Consortium. 2022. Engineering Biology for Climate and Sustainability: A research roadmap for a cleaner future.
  3. Sagues, W.J. and A. Woodley. 2022. Building a Biotechnology Innovation Ecosystem to Mitigate Climate Change.
  4. Buan, N.R. 2022. Course Portfolio for BIOC934: Genome Dynamics and Gene Expression. Department of Biochemistry, University of Nebraska-Lincoln.